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Use Cases and Science Data

Use Cases and Science Data

At Supplements4Muscle we stock only those sports-nutrition formulas that are supported by reproducible data and manufactured in GMP-certified facilities. Whether you are a competitive athlete, a seasoned gym-goer or a weekend warrior, understanding what a supplement can and cannot do is the first step toward responsible use. This page summarises the current peer-reviewed science on nine of our best-selling product categories:

  • Prohormones
  • Selective Androgen Receptor Modulators (SARMs)
  • Testosterone boosters
  • Creatine
  • Protein (whey, casein & plant blends)
  • Liver-support formulas
  • Fat burners / thermogenics
  • Pre-workout products
  • Amino acids & peptide-based ergogenics

For every group you will find: 

• What it is 

• How it works (mechanism of action) 

• Documented benefits 

• Possible risks & side effects 

• Direct links to high-quality scientific sources

Nothing here is a substitute for medical advice. Always consult a qualified healthcare professional before introducing a new supplement, particularly if you compete in drug-tested sport, take prescription medication or have pre-existing medical conditions.

PROHORMONES – SCIENTIFIC OVERVIEW 

What are prohormones? 

Synthetic precursors that are enzymatically converted in vivo into active anabolic-androgenic hormones such as testosterone, nandrolone or trenbolone analogues. Although originally marketed as a “legal” alternative to steroids, most first-generation prohormones were placed on controlled-substance lists between 2004 and 2014.

How do they work? 

• Oral absorption → hepatic 3β-HSD / 17β-HSD conversion → active hormone 

• The newly formed hormone binds to cytosolic androgen receptors → DNA transcription → ↑ muscle-protein synthesis, ↑ satellite-cell activation, ↓ myostatin expression.

Benefits (documented in resistance-trained adults) 

• 2-7 kg increase in lean body mass over 4-8 weeks (Brown et al., 2004) 

• 5-20 % strength improvement in multi-joint lifts when combined with structured training • Faster recovery via ↓ creatine-kinase (CK) and ↓ muscle-soreness markers.

Risks & side effects 

• Endogenous testosterone suppression → post-cycle hypogonadism, reduced libido 

• Hepatotoxicity for 17-α-alkylated molecules (elevated ALT/AST) 

• Adverse lipid profile (↓ HDL, ↑ LDL), potential ↑ blood-pressure.

Key papers 

• Brown GA et al. “Effects of prohormone supplementation in humans.” Clin J Sport Med 2004. 

• Schulze JJ et al. “Testosterone prohormone supplements.” Eur J Endocrinol 2006.

Disclaimer: Prohormones are banned by WADA, IOC and most federations.

SARMs – SCIENTIFIC OVERVIEW 

What are SARMs? 

Non-steroidal molecules that selectively activate androgen receptors in skeletal muscle and bone while sparing reproductive organs and skin.

Mechanism of action 

• High affinity for the AR ligand-binding domain in myocytes → anabolic gene transcription. 

• Minimal 5-α-reduction to DHT → reduced prostate stimulation; minimal aromatisation to oestrogens.

Benefits 

• Lean-mass gains of 1-3 kg in 12 weeks at therapeutic doses (Gundersen et al., 2020). 

• Ostarine (MK-2866) ↑ bone-mineral density and ↓ hip-fracture risk in elderly models. 

• Cardarine (GW-501516 – technically a PPARδ agonist but often grouped with SARMs) ↑ fatty-acid oxidation and endurance performance.

Risks 

• Moderate LH/FSH suppression after 4+ weeks; PCT often required. 

• Sporadic reports of hepatotoxicity (liver-enzyme elevations return to baseline after discontinuation). 

• Long-term carcinogenicity and cardiovascular safety still under investigation.

Key papers 

• Narayanan R et al. “SARMs as function-promoting therapies.” Curr Opin Clin Nutr Metab Care 2009.

• US Pharmacist. “Recreational use of SARMs.” 2017.

Disclaimer: No SARM is FDA-approved for human use; all major anti-doping bodies prohibit them.

TESTOSTERONE BOOSTERS – SCIENTIFIC OVERVIEW 

Definition 

Botanical extracts, amino acids, minerals and vitamins that aim to optimise endogenous testosterone production in individuals whose levels are low-normal rather than clinically deficient.

Mechanisms 

• Hypothalamic stimulation (D-aspartic acid, Mucuna pruriens → ↑ GnRH) 

• Aromatase inhibition (zinc, boron) → ↓ oestrogen feedback • SHBG reduction (fenugreek saponins) → ↑ free testosterone 

• Sleep-quality support (magnesium, ashwagandha) → ↑ nocturnal testosterone pulses.

Benefits

• Men with baseline low-normal T (<400 ng/dL) may see 10-25 % increases within 2-8 weeks. 

• Associated improvements in libido, mood and perceived vitality. 

• Potential small but meaningful increases in strength (2-5 %) if combined with resistance training.

Risks & caveats 

• Minimal side effects (mild GI upset or acne) at recommended doses. 

• Limited efficacy when baseline testosterone is already optimal (>600 ng/dL). 

• Some herbs (tribulus, tongkat ali) may interact with antidepressants or antihypertensives.

Key papers 

• Topo E et al. “The role and molecular mechanism of D-aspartic acid…” Reprod Biol Endocrinol 2009 

• Wankhede S et al. “Examining the effect of fenugreek extract on strength and body composition.” J Sport Health Sci 2016. 

CREATINE – SCIENTIFIC OVERVIEW 

What is creatine? 

A naturally occurring guanidino compound synthesised from arginine, glycine and methionine; stored as phosphocreatine in muscle and brain tissue.

How it works 

• ↑ Phosphocreatine stores → faster ATP resynthesis during high-intensity effort. 

• Cell-volumising effect → triggers anabolic signalling pathways (mTOR, MAPK). 

• Neuroprotective and myoprotective actions via antioxidant mechanisms.

Benefits (supported by >1 000 studies) 

• +5-15 % performance in repeated sprint / heavy lift activities.

 • 1-3 kg lean-mass gain over 4 weeks largely from intracellular water & protein synthesis. 

• Cognitive benefits: improved memory/processing speed in sleep-deprived subjects.

Risks & side effects 

• Generally safe; the ISSN classifies creatine monohydrate as “the most effective ergogenic aid available to athletes.” 

• Transient weight gain, mild stomach cramp if inadequately hydrated. 

• No evidence of kidney damage in healthy individuals (Poortmans & Francaux, 1999).

Key papers 

• Kreider RB et al. “International Society of Sports Nutrition position stand: creatine.” JISSN 2017. 

• Rawson ES, Venezia AC. “Use of creatine in the elderly…” Curr Aging Sci 2011. 

PROTEIN SUPPLEMENTS – SCIENTIFIC OVERVIEW 

What are they? 

Concentrated food-derived proteins (whey, casein, egg, beef, pea, rice) processed into powders for convenient ingestion.

Mechanisms 

• Provide essential amino acids, especially leucine, which triggers mTOR-dependent muscle-protein synthesis (MPS). 

• Whey: rapid digestion → spike in plasma amino acids → acute MPS. 

• Casein: slow release → anti-catabolic during overnight fasts.

Benefits 

• Meta-analysis shows additional 0.5-1.0 kg lean-mass gain and 20–35 % greater strength when ≥1.6 g protein/kg bw/day is achieved (Morton et al., 2018). 

• Improved satiety and body-fat management. 

• Immunoglobulins and lactoferrin in native whey may support immune health.

Risks & considerations 

• Lactose intolerance → choose isolate or hydrolysate. 

• Excessive intake (>3 g/kg) offers no extra benefit and may cause GI discomfort. 

• No deleterious effect on kidney function in healthy individuals.

Key papers 

• Morton RW et al. “Protein supplementation to augment resistance training…” Br J Sports Med 2018.

• Phillips SM. “A brief review of higher dietary protein diets…” Ann Nutr Metab 2016.

LIVER-SUPPORT SUPPLEMENTS

What are they?

Herbal extracts (milk thistle, artichoke, turmeric/curcumin), phospholipids (phosphatidylcholine), antioxidants (NAC, SAMe) formulated to protect hepatocytes and enhance phase I/II detoxification.

Mechanisms 

• Silymarin (milk-thistle) stabilises hepatocyte membranes, scavenges free radicals. 

• N-acetyl-cysteine replenishes glutathione, the body’s master antioxidant.

• Curcumin modulates NF-κB → ↓ inflammatory cytokines.

Benefits 

• 30-50 % reduction in ALT/AST in patients with mild non-alcoholic fatty-liver disease (Loguercio et al., 2012). 

• Potentially mitigates hepatotoxic stress from oral anabolics or high-protein diets. 

• Improved bile flow and digestion.

Risks & side effects 

• Generally well tolerated; high-dose curcumin may cause mild GI discomfort. 

• NAC may interact with nitroglycerine (hypotension). 

• Always monitor liver enzymes during any hepatotoxic drug/supplement regimen.

Key papers 

• Loguercio C et al. “The effect of a silybin-vitamin E-phospholipid complex in NAFLD.” World J Gastroenterol 2012.  

• Nuttall SL et al. “Glutathione: in sickness and in health.” Lancet 1998. 

FAT BURNERS / THERMOGENICS

Definition 

Formulas aimed at increasing energy expenditure, mobilising stored fat or suppressing appetite. Typical ingredients: caffeine, green-tea catechins (EGCG), synephrine, yohimbine, capsaicin and L-carnitine.

Mechanisms 

• Sympathomimetic stimulation (caffeine, synephrine) → ↑ catecholamines → ↑ lipolysis. 

• Mitochondrial uncoupling (capsaicin) → ↑ thermogenesis. 

• Alpha-2 adrenergic antagonism (yohimbine) → enhanced stubborn-fat mobilisation. 

• Carnitine facilitates fatty-acid transport into mitochondria; effect more pronounced in deficient or vegan athletes.

Benefits 

• Acute metabolic-rate increase of 3-11 % with 200-400 mg caffeine (Astrup et al., 1990). 

• 0.5-1.5 kg extra fat loss over 8-12 weeks when combined with caloric deficit and exercise. 

• Enhanced training intensity via central-nervous-system stimulation.

Risks & side effects 

• Elevated heart rate, blood pressure, potential anxiety/insomnia. 

• Yohimbine may cause panic attacks in sensitive individuals. 

• Tolerance develops; cycling is recommended.

Key papers 

• Dulloo AG et al. “Green tea and thermogenesis.” Am J Clin Nutr 1999.

• Astrup A et al. “Caffeine and coffee: effect on metabolic rate…” Am J Clin Nutr 1990.

PRE-WORKOUT PRODUCTS 

What are they?

Multi-ingredient blends delivering stimulants, nitric-oxide boosters, nootropics and electrolytes 20-30 minutes before exercise.

Key actives & mechanisms

• Caffeine + theanine → synergistic alertness without jitters. 

• Beta-alanine → ↑ muscle carnosine → buffering of H+ → delayed fatigue (tingling is harmless paraesthesia). 

• Citrulline malate / arginine → ↑ plasma L-arginine → ↑ nitric oxide → vasodilation and nutrient delivery. 

• Tyrosine & choline donors → support catecholamine synthesis and focus.

Benefits 

• +12-16 % average improvement in muscular endurance (Hoffman et al., 2008). 

• Enhanced subjective energy, focus, and training volume leading to greater hypertrophy over time.

Risks & side effects 

• Over-stimulation, insomnia if taken late in the day. 

• Niacin flush, paresthesia from beta-alanine. • Beware of proprietary blends that hide exact caffeine amounts; avoid combining with other stimulants.

Key papers 

• Trexler ET et al. “International Society of Sports Nutrition position stand: beta-alanine.” JISSN 2015. 

• Gonzalez AM et al. “Citrulline supplementation…” J Strength Cond Res 2018. 

AMINO ACIDS & PEPTIDE ERGOGENICS 

What are they?

Isolated essential amino acids (EAAs), branched-chain amino acids (BCAAs), conditionally essential aminos (glutamine, arginine) and novel peptides such as collagen or velositol.

Mechanisms 

• Leucine acts as a trigger for MPS via mTOR. 

• BCAAs reduce central fatigue by competing with tryptophan for transport across the blood-brain barrier. 

• L-citrulline converts to arginine → ↑ nitric oxide. 

• Collagen peptides stimulate fibroblast activity → improved joint/tendon health.

Benefits

 • 6 g EAAs post-workout can elevate MPS similarly to 25 g whey for 2-3 h (Tipton et al., 2007). 

• 10 g collagen with vitamin C twice daily improved knee-joint pain in athletes (Clark et al., 2008). • In endurance sports, BCAAs may lower perceived exertion and DOMS.

Risks & side effects 

• Very safe; excessive isolated BCAA intake without the other EAAs could theoretically reduce net protein balance. 

• Individuals with maple-syrup-urine disease (rare) must avoid BCAAs.

Key papers 

• Tipton KD et al. “Stimulation of net muscle protein synthesis by whey protein…” Am J Physiol 2007. https://pubmed.ncbi.nlm.nih.gov/17218495/ 

• Clark KL et al. “24-week study on the use of collagen hydrolysate…” Curr Med Res Opin 2008. https://pubmed.ncbi.nlm.nih.gov/18416885/

Train hard, recover smart, and supplement responsibly.